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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Complement C3 Antibody (aa1580‑1592) IHC‑plus™ LS‑B11161
Note: This antibody replaces LS-C155064
Complement C3 antibody LS-B11161 is an unconjugated goat polyclonal antibody to Complement C3 from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11161-50
50 µg (0.5 mg/ml)
Unavailable

Popular Complement C3 Products

Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Flow Cytometry, ELISA
Mouse thymocytes incubated with Rabbit Anti-Mouse T cells, then incubated with fresh human serum and stained with LS-C62855. Antibody concentration: 0.5ug/10^6 cells.. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Flow Cytometry
Anti-Complement C3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2545 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Complement C3 antibody LS-B11161 is an unconjugated goat polyclonal antibody to Complement C3 from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About Complement C3
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. P01024 NM_000064 NP_000055.2

C3 Antibody, AHUS5 Antibody, ARMD9 Antibody, Complement component C3 Antibody, CPAMD1 Antibody, Complement C3 Antibody, ASP Antibody, Complement component 3 Antibody


Specifications

Target
Human Complement C3
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Complement C3 antibody was raised against synthetic peptide QQRTFISPIKCRE from the C-terminus of human Complement C3 (NP_000055.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%).
Blocking Peptide
LS-E27967 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11161. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1580-1592
Specificity
Human Complement C3. This antibody is expected to recognize the C3 alpha chain, C3b alpha chain and the C3c alpha chain fragment 2.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.
Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.
Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.
Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Complement C3 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.
Anti-Complement C3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11161 concentration 5 ug/ml.

Requested From: 
Date Requested: 7/16/2018

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