Human Complement C3
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Complement C3 antibody was raised against synthetic peptide QQRTFISPIKCRE from the C-terminus of human Complement C3 (NP_000055.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11161. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human Complement C3. This antibody is expected to recognize the C3 alpha chain, C3b alpha chain and the C3c alpha chain fragment 2.
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide.
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.