Human Complement C3

Goat

Human, Monkey (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence QQRTFISPIKCRE, from the C-Terminus of protein sequence according to NP_000055.2.

aa1580-1592

Human Complement C3. This antibody is expected to recognize the C3 alpha chain, C3b alpha chain and the C3c alpha chain fragment 2.

• IHC
• IHC - Paraffin (5 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01024 NCBI: NM_000064 NP_000055.2