Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:50)
Western blot (1:500)
Specificity and Use
Collagen IV antibody was raised against human amniotic type IV collagen.
Recognizes human type IV collagen, alpha 2 chains. Species cross-reactivity: Monkey and rat.
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Immunohistochemistry: 1:250;. Weak reactivity on on formalin fixed, paraffin sections at 1:100 after treatment with 1% trypsin for 20 minutes at RT. recommended. Western Blot: 1:250; Detects 170kD band. ELISA: 1:100.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.
Anti-Collagen IV antibody IHC of human kidney, basement membrane. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7348 dilution 1:50.