(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
- IHC - Frozen (1:20)
- Dot Blot
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Collagen IV antibody was raised against human Collagen IV.
Recognizes human Collagen IV. Minimal cross-reactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.
Suitable for use in Immunohistochemistry, ELISA and Dot Blot. Immunohistochemistry (Frozen): 1:20 if Peroxidase labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin. ELISA: Native human collagen IV. Dot Blot: Native human collagen IV.
1,000 µl Distilled water
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 6 months at -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.