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Anti-Collagen IV Antibody IHC-plus™ LS-B2717

Note: This antibody replaces LS-C87413
Catalog Size Price
LS-B2717-50 50 µg (1 mg/ml) Unavailable

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Anti-Collagen IV Antibody IHC-plus™ LS-B2717
Rabbit Polyclonal to Human Collagen IV
Human, Bovine
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Collagen IV Antibody IHC-plus™ LS-B3654
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Anti-Collagen IV Antibody (clone MC4-HA) LS-C121649
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Anti-Collagen IV Antibody IHC-plus™ LS-B8763
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Immunohistochemistry Image
Anti-Collagen IV Antibody IHC-plus™ LS-B8976
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Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human Collagen IV
Human, Bovine
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human Collagen IV
Rabbit
Human, Bovine (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

Collagen Type IV from human and bovine placenta.
Immunohistochemistry: LS-B2717 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2717 was determined to be 2.5 ug/ml.

Packaging

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Store vial at 4 C prior to opening. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20 C or below.
For research use only.

About Collagen IV

Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen IV antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2717 concentration 2.5 ug/ml.

Requested From: 
Date Requested: 10/18/2017

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