Mammal Collagen IV

Rabbit

Mammal, Human, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Collagen Type IV from human and bovine placenta.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:1000 - 1:10000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:50000)

Immunohistochemistry: LS-B2717 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2717 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

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