Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Frozen, ICC, Immunofluorescence, ELISA, Dot Blot
Human Collagen IV
Human (tested or 100% immunogen sequence identity)
IHC - Frozen (1:20)
Specificity and Use
Collagen IV antibody was raised against human collagen type IV.
Recognizes human Collagen IV. Minimal cross-reactions with other collagens were only found if 20-50 times higher concentrations were used of other collagens.
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Immunohistochemistry (Frozen): 1:20 if Peroxidase labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin. ELISA: Native human collagen IV. Dot Blot: Native human collagen IV.
1,000 µl Distilled water
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C
For research use only.
About Collagen IV
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.