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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen IV Antibody LS‑C121653
Antibody
Collagen IV antibody LS-C121653 is an unconjugated rabbit polyclonal antibody to human Collagen IV. Validated for DB, ELISA, ICC, IF and IHC.
Catalog
Size
Price
LS-C121653-1000
1000 µl
Unavailable

Popular Collagen IV Products

Anti-Collagen IV antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2717 concentration 2.5 ug/ml.
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Anti-Collagen IV antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3654 dilution 1:100.
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Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ELISA
Anti-Collagen IV antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat, Cat, Dog, Pig, Fish
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Human, Kidney, glomerulus: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot

Product Description

Collagen IV antibody LS-C121653 is an unconjugated rabbit polyclonal antibody to human Collagen IV. Validated for DB, ELISA, ICC, IF and IHC.
About Collagen IV
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.

Specifications

Target
Human Collagen IV
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1:20)
  • ICC
  • Immunofluorescence
  • ELISA
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Collagen IV antibody was raised against human collagen type IV.
Specificity
Recognizes human Collagen IV. Minimal cross-reactions with other collagens were only found if 20-50 times higher concentrations were used of other collagens.
Usage
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Immunohistochemistry (Frozen): 1:20 if Peroxidase labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin. ELISA: Native human collagen IV. Dot Blot: Native human collagen IV.
Presentation
Lyophilized
Reconstitution
1,000 µl Distilled water
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 6 months at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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