Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1043] (IgG1) to Human Collagen IV
IHC - Frozen, ICC, Western blot
Human Collagen IV
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal 
IHC - Frozen (1:50 - 1:100)
Western blot (1:100 - 1:1000)
IHC - Paraffin
Specificity and Use
Collagen IV antibody was raised against type IV collagen isolated from human placenta
Recognizes the extracellular, basement membranes.
Methanol fixed cells are recommended for immunocytochemistry. A PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended for immunohistochemistry on frozen tissues. For immunohistochemistry on paraffin tissue sections, the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.
PBS containing 0.09% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.
About Collagen IV
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.