Chicken Collagen II

Mouse

Chicken, Human, Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Purified

Unmodified

Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.

Recognizes both alpha 1 (II) and alpha 3 (XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Species cross-reactivity: Human, Chicken, Mouse, Salamander.

• IHC
• IHC - Paraffin (1:200)
• Western blot (1:200 - 1:400)
• ELISA

In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Western Blot of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA. Western Blot: 1:200-1:400. Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 1-2 ug/ml. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1 mg/ml Tris HCl, pH 2 for 15 minutes at RT or 15 minutes at 37?C.

10 mM PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA

For research use only. Intended for use by laboratory professionals.

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