(tested or 100% immunogen sequence identity)
- IHC - Frozen
- Western blot (1:5000 - 1:10000)
- ELISA (1:10000 - 1:50000)
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Collagen II antibody was raised against collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Recognizes human Collagen Type II. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- fixed, paraffin embedded tissues. Recognizes most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:5000-1:10000. ELISA: 1:10000-1:50000. Immunohistochemistry (frozen): 1:200-1:1000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.125. M sodium borate, 0.075 M sodium chloride, 10 mg/ml BSA (IgG, protease free), 0.005 M EDTA 0.1% sodium azide.
For research use only.
This antibody carries the LSBio 100% Guarantee.