Human Collagen I

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Ammonium sulfate precipitation

Unmodified

Human placental collagen Type I.

Human collagen type. Cross-reactivity: Human collagen type I: 100%, Human collagen type II: <0.5%, Human collagen type III: < 25%, Human collagen type IV: <0.5%, Human collagen type V: <0.5%, Human fibronectin: <0.5%, Human albumin: <0.5%, Human immunoglobulin: <0.5%.

• IHC
• IHC - Paraffin (1:100)
• Immunofluorescence
• ELISA

• Western blot

Immunohistochemistry: LS-B3653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

PBS, pH 7.5, 0.1% Mannitol, 0.1% Dextran.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.