Human Collagen I

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Ammonium sulfate precipitation

Unmodified

Human Collagen Type I.

Recognizes both native and heat denatured human collagen type I. No cross-reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and III in ELISA, RIA or immunoblot.

• IHC
• IHC - Frozen
• ELISA (10 - 20 µg/ml)
• Radioimmunoassay

• IHC - Paraffin
• Western blot

Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20 ug/ml, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldehyde fixation.

Lyophilized

ddH2O or 40-50% glycerol

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.