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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen I Antibody LS‑C79499
Antibody
Collagen I antibody LS-C79499 is an unconjugated rabbit polyclonal antibody to salmon Collagen I. Validated for ELISA, IF and IHC.
Catalog
Size
Price
LS-C79499-500
500 µl
Unavailable

Popular Collagen I Products

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Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Product Description

Collagen I antibody LS-C79499 is an unconjugated rabbit polyclonal antibody to salmon Collagen I. Validated for ELISA, IF and IHC.
About Collagen I
Type I collagen is the most abundant collagen of the human body which forms large, eosinophilic fibers known as collagen fibers. It is present in scar tissue, the end product when tissue heals by repair, as well as tendons, ligaments, the endomysium of myofibrils, the organic part of bone, the dermis, the dentin and organ capsules. Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is encoded by the COL1A1 gene. Collagen alpha-2(I) chain is encoded by the COL1A2 gene.

Specifications

Target
Salmon Collagen I
Host
Rabbit
Reactivity
Salmon (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunofluorescence
  • ELISA (1:10 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Collagen I antibody was raised against collagen Type I extracted and purified from sh skin.
Specificity
Recognizes salmon Collagen Type I. Crossreactivity: OD at a 1:100 ELISA dilution: Salmon fish Collagen Type 1: >1; Sole and tuna fish collagen type I: <0.4; Goldfish Collagen Type I: <0.2; Mammalian, avian Collagen Type I: <0.2. Note: OD <0.2 means negative reactivity.
Usage
Suitable for use in IFA, SPRIA, and ELISA. IFA (indirect): 1:40 on frozen salmon tissues (skin, liver). Suitable for immunostaining of extra- or intracellular components in light microscopy in conjunction with anti-rabbit IgG-fluorescein conjugate. ELISA: 1:10-1:200.
Presentation
Lyophilized powder. No preservative added. Reconstitute, 0.5 ml sterile ddH2O or 40-50% glycerol.
Reconstitution
500 µl ddH2O or 40-50% glycerol
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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