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Anti-Collagen I Antibody IHC-plus™ LS-B3653

Note: This antibody replaces LS-C79515

Ordering

Wt. Vol. Conc. Price
- 125 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Collagen I Antibodies

Anti-Collagen I Antibody IHC-plus™ LS-B342
Rabbit Polyclonal to Human Collagen I
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Collagen I Antibody IHC-plus™ LS-B3653
Rabbit Polyclonal (IgG) to Human Collagen I
Human
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Collagen I Antibody (clone COL-1) IHC-plus™ LS-B6723
Mouse Monoclonal [clone COL-1] (IgG1) to Bovine Collagen I
Bovine, Human, Rat, Pig, Rabbit, Deer
IHC - Paraffin, IHC - Frozen, IHC - 4% paraformaldehyde, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human Collagen I
Human
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated

Details

Human Collagen I
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Immunofluorescence
  • ELISA

  • Western blot

Specificity and Use

Collagen I antibody was raised against human placental collagen Type I.
Human collagen type. Cross-reactivity: Human collagen type I: 100%, Human collagen type II: <0.5%, Human collagen type III: < 25%, Human collagen type IV: <0.5%, Human collagen type V: <0.5%, Human fibronectin: <0.5%, Human albumin: <0.5%, Human immunoglobulin: <0.5%.
Immunohistochemistry: LS-B3653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

Packaging

Lyophilized, PBS, pH 7.5, 0.1% Mannitol, 0.1% Dextran.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Collagen I

Type I collagen is the most abundant collagen of the human body which forms large, eosinophilic fibers known as collagen fibers. It is present in scar tissue, the end product when tissue heals by repair, as well as tendons, ligaments, the endomysium of myofibrils, the organic part of bone, the dermis, the dentin and organ capsules. Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is encoded by the COL1A1 gene. Collagen alpha-2(I) chain is encoded by the COL1A2 gene.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen I antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3653 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen I antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3653 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen I antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3653 dilution 1:100.
 

Requested From: United States
Date Requested: 12/5/2016

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