Human Collagen I

Mouse

Human, Sheep (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Human collagen.

C-Terminus

Recognizes Collagen Type I near the C-terminal. Displays a high affinity for human, bovine and sheep Type I Collagens. There is no evidence for cross-reactivity with Collagen Types III, V and VI or connective tissue proteins (Elastin, Fibronectin and Laminin) at suggested working concentrations. Species cross-reactivity: Human bovine, sheep, porcine and canine. Does not react with mouse, rat, guinea pig, squirrel, equine, kangaroo or chicken.

• IHC
• IHC - Frozen
• Western blot
• Flow Cytometry (1:100)
• ELISA (1:50 - 1:250)

Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, and Western Blot. ELISA (Capture): 2-4 ug/ml. ELISA (Detection): 10 ug/ml will detect 50 ng of human Collagen Type I with no reactivity with other Collagens. Higher levels of antibody may be necessary to detect lower concentrations of Collagen. Immunohistochemistry (Unfixed frozen sections): 1:50-1:250. Flow Cytometry: 1:100. Western Blot (non-denatured and non-reduced): 1:500. Positive control: Human kidney lysate.

50 mM Tris, pH 4.5, 150 mM NaCl, 0.1% Bronidox

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.