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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen I Antibody (Biotin) LS‑C79501
Collagen I antibody LS-C79501 is a biotin-conjugated goat polyclonal antibody to human Collagen I. Validated for ELISA, ICC, IHC and WB.
Antibody
Catalog
Size
Price
LS-C79501-200
200 µg
Unavailable

Popular Collagen I Products

Anti-Collagen I antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B209 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Product Description

Collagen I antibody LS-C79501 is a biotin-conjugated goat polyclonal antibody to human Collagen I. Validated for ELISA, ICC, IHC and WB.
About Collagen I
Type I collagen is the most abundant collagen of the human body which forms large, eosinophilic fibers known as collagen fibers. It is present in scar tissue, the end product when tissue heals by repair, as well as tendons, ligaments, the endomysium of myofibrils, the organic part of bone, the dermis, the dentin and organ capsules. Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is encoded by the COL1A1 gene. Collagen alpha-2(I) chain is encoded by the COL1A2 gene.

Specifications

Target
Human Collagen I
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Western blot
  • ELISA (1:1000 - 1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Collagen I antibody was raised against human Type I collagen.
Specificity
Reacts with conformational determinants on human type I collagen as demonstrated by ELISA. May react with type I collagen from other species. Exhibits < 10% cross-reactivity with collagen type II, III, IV, V and VI. Has not been tested for reactivity with other ECM proteins (e. g., laminin, fibronectin).
Usage
Enzyme-Linked-Immunosorbent-Assay (ELISA). Immunocytochemistry. Dot- and slot-immunoblotting. Immunohistochemistry (frozen sections). Immunocytochemistry/Immunohistochemistry: 1:20-1:40. ELISA: 1:1000-1:4000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, sodium azide.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/19/2018

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