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Anti-COL25A1 / Collagen XXV Antibody (aa155-169) IHC-plus™ LS-B663

Note: This antibody replaces LS-C2633, LS-C79371, LS-C22239, LS-C22238

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular COL25A1 / Collagen XXV Antibodies

Anti-COL25A1 / Collagen XXV Antibody (aa171-183) LS-C2632
Rabbit Polyclonal to Human COL25A1 / Collagen XXV
Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Anti-COL25A1 / Collagen XXV Antibody (aa155-169) IHC-plus™ LS-B663
Rabbit Polyclonal to Human COL25A1 / Collagen XXV
Human, Monkey, Mouse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-COL25A1 / Collagen XXV Antibody (aa430-443) IHC-plus™ LS-B664
Rabbit Polyclonal to Human COL25A1 / Collagen XXV
Human, Monkey, Mouse, Bat, Bovine, Dog, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human COL25A1 / Collagen XXV
Human, Monkey, Mouse, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Human COL25A1 / Collagen XXV
Rabbit
Human, Monkey, Mouse, Rabbit (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500)

Specificity and Use

COL25A1 / Collagen XXV antibody was raised against a synthetic peptide mapping to residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rabbit (100%); Opossum, Xenopus (93%); Zebrafish (80%).
aa155-169
Reacts with residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kD in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy.
Immunohistochemistry: LS-B663 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B663 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About COL25A1 / Collagen XXV

Q9BXS0 NM_032518 NP_115907.2

COL25A1 Antibody, AMY Antibody, CLAC-P Antibody, CLAC Antibody, CLACP Antibody, Collagen, type XXV, alpha 1 Antibody, Collagen alpha-1(XXV) chain Antibody

COL25A1 / Collagen XXV is a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410).

Immunohistochemistry

Immunohistochemistry
Anti-COL25A1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B663 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-COL25A1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B663 concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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