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Anti-Endostatin Antibody IHC-plus™ LS-B8215

Note: This antibody replaces LS-C104551

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Endostatin Antibody LS-B8215 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Endostatin antibody IHC of human small intestine.
Western blot
Western Blot (non-reducing) of Endostatin antibody LS-B8215
Western blot
Western Blot (reducing) of Endostatin antibody LS-B8215
Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Endostatin antibody LS-B8215

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Rabbit Polyclonal to Human Endostatin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Endostatin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.2 µg/ml), ELISA (0.5 - 2 µg/ml)

Specificity and Use

Endostatin antibody was raised against e.coli derived recombinant Human Endostatin.
Human Endostatin
Immunohistochemistry: LS-B8215 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hEndostatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Endostatin (LS-C104550) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEndostatin. Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About Endostatin

P39060 NM_030582 NP_085059.2

COL18A1 Antibody, Collagen, type XVIII, alpha 1 Antibody, Collagen alpha-1(XVIII) chain Antibody, Endostatin Antibody, KNO Antibody, KNO1 Antibody, KS Antibody, Multi-functional protein MFP Antibody, Antiangiogenic agent Antibody, Collagen alpha 1(xviii) Antibody, Knobloch syndrome, type 1 Antibody

Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors.

Immunohistochemistry

Immunohistochemistry
Anti-Endostatin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8215 dilution 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of Endostatin antibody LS-B8215

Western blot

Western blot
Western Blot (reducing) of Endostatin antibody LS-B8215

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Endostatin antibody LS-B8215

Requested From: United States
Date Requested: 8/4/2015

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