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Anti-CML / BCR Antibody (clone 2E5) LS-C196741

Catalog Size Price
LS-C196741-100 100 µg Unavailable

Related Products

43 CML / BCR Antibodies

Most Popular CML / BCR Antibodies

Anti-CML / BCR Antibody (aa50-100) LS-C288812
Rabbit Polyclonal (IgG) to Human CML / BCR
Human
ICC, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Proximity Ligation Assay
Unconjugated
Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
Anti-CML / BCR Antibody (aa1221-1271) LS-C288817
Rabbit Polyclonal (IgG) to Human CML / BCR
Human, Mouse, Rat, Dog, Chicken, Xenopus, Zebrafish
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Proximity Ligation Assay
Unconjugated
Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
Anti-CML / BCR Antibody (phospho-Tyr360) LS-C358919
Rabbit Polyclonal to Human CML / BCR
Human, Monkey, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of BCR (pY360) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-CML / BCR Antibody (phospho-Tyr360) LS-C381491
Rabbit Polyclonal (IgG) to Human CML / BCR
Human, Monkey, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-CML / BCR Antibody (phospho-Tyr360) LS-C416534
Rabbit Polyclonal to Human CML / BCR
Human, Mouse
IHC, ICC, Immunofluorescence, Western blot
Unconjugated
Western blot of Bcr phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

100% Guaranteed
Mouse Monoclonal [clone 2E5] (IgG2a,k) to Human CML / BCR
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CML / BCR
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2E5]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

CML / BCR antibody was raised against bCR (NP_004318.3, aa182-280 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CML / BCR
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CML / BCR

P11274 NM_004327 NP_004318.3

BCR Antibody, Acute lymphocytic leukemia Antibody, ALL Antibody, BCR1 Antibody, BCR/FGFR1 chimera protein Antibody, Breakpoint cluster region Antibody, Chronic myeloid leukemia Antibody, D22S11 Antibody, CML Antibody, D22S662 Antibody, Philadelphia chromosome Antibody, PHL Antibody, FGFR1/BCR chimera protein Antibody

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint.

Immunofluorescence

Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/19/2018

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