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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CML / BCR Antibody (clone 2E5) LS‑C196741
BCR antibody LS-C196741 is an unconjugated mouse monoclonal antibody to human BCR (CML). Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C196741-100
100 µg
Unavailable

Popular CML / BCR Products

IHC of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The sample on the right was incubated with synthetic peptide.
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Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
Species: Human, Mouse, Rat, Dog, Chicken, Xenopus, Zebrafish
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Proximity Ligation Assay
Immunohistochemical analysis of BCR (pY360) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human, Monkey, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
Western blot of Bcr phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot

Product Description

BCR antibody LS-C196741 is an unconjugated mouse monoclonal antibody to human BCR (CML). Validated for ELISA, IF and WB.
About CML / BCR
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. P11274 NM_004327 NP_004318.3

BCR Antibody, Acute lymphocytic leukemia Antibody, ALL Antibody, BCR1 Antibody, BCR/FGFR1 chimera protein Antibody, Breakpoint cluster region Antibody, Chronic myeloid leukemia Antibody, D22S11 Antibody, CML Antibody, D22S662 Antibody, Philadelphia chromosome Antibody, PHL Antibody, FGFR1/BCR chimera protein Antibody


Specifications

Target
Human CML / BCR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2E5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
CML / BCR antibody was raised against bCR (NP_004318.3, aa182-280 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CML / BCR
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunofluorescence

Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BCR on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BCR expression in transfected 293T cell line by BCR monoclonal antibody (M01), clone 2E5.Lane 1: BCR transfected lysate (Predicted MW: 121 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCR is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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