Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone B7] (IgG1,k) to Human CMA1 / Mast Cell Chymase
Human CMA1 / Mast Cell Chymase
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [B7]
Ion exchange chromatography
IHC (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Specificity and Use
Chymase purified from human skin.
Recognizes human mast cell chymase.
Suitable for use in Immunohistochemistry and Immunocytochemistry. Not recommended for Western Blot. Immunohistochemistry/Immunocytochemistry: 0.5-1 ug/ml; For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. Positive control: Lung, Heart, Skin, Placenta. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.1, 0.09% sodium azide, 15 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
CMA1 / MCT1 / Chymase is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.