Human CLU / Clusterin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Native clusterin purified from human serum.

Recognizes human clusterin, alpha at 40kD, and precursor protein.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.5 - 2 µg/ml)

Immunohistochemistry: LS-B2218 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2218 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 40%

Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10909 NCBI: NM_001831 NP_001822.3