Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Human CLU / Clusterin
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin
Western blot (1:2500 - 1:5000)
ELISA (1:2000 - 1:4000)
Specificity and Use
CLU / Clusterin antibody was raised against recombinant Apolipoprotein J.
Recognizes human Apolipoprotein J. Negligible cross-reactivity to Apo A-I, A-II, B, C-I, C-II, C-III and E by ELISA. Negligible non-specific cross-reaction with other human serum proteins.
Suitable for use in Western Blot, Immunohistochemistry, ELISA and Immunoprecipitation. Western Blot: 1:2500-1:5000. Immunohistochemistry (Paraffin): 1:25-1:100. ELISA: 1:2000-1:4000; Standard sandwich assay.
0.125 M sodium borate buffer, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself.