Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)
Specificity and Use
CLU / Clusterin antibody was raised against e. coli-derived human Apolipoprotein J recombinant protein (Position: S228-E449). Human Apolipoprotein J shares 76.1 % and 75.2 % amino acid (aa) sequence identity with mouse and rat Apolipoprotein J, respectively. Percent identity by BLAST analysis: Human (100%); Mouse, Rat (58%).
Reacts with: human CLU / Clusterin.
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitute with 200 µl of distilled water.
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4 °C or long term at -20 °C or below. Avoid freeze/thaw cycles.
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself.
Apolipoprotein J antibody IHC-paraffin. IHC(P): Human Testis Tissue.
Apolipoprotein J antibody Western blot. All lanes: Anti Apolipoprotein J at 0.5 ug/ml. Lane 1: SKOV Whole Cell Lysate at 40 ug. Lane 2: U87 Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Predicted band size: 52 kD. Observed band size: 82 kD.