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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CLU / Clusterin Antibody (aa1‑333, clone 1A11) IHC‑plus™ LS‑B2937
Note: This antibody replaces LS-C93726
Clusterin antibody LS-B2937 is an unconjugated mouse monoclonal antibody to human Clusterin (CLU). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2937-50
50 µg (1 mg/ml)
Unavailable

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Antibody
Species: Human
Applications: ELISA

Product Description

Clusterin antibody LS-B2937 is an unconjugated mouse monoclonal antibody to human Clusterin (CLU). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CLU / Clusterin
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. P10909 NM_001831 NP_001822.3

CLU Antibody, APO-J Antibody, Apolipoprotein J Antibody, CLU1 Antibody, Clusterin Antibody, Complement lysis inhibitor Antibody, CLI Antibody, AAG4 Antibody, Aging-associated protein 4 Antibody, Ku70-binding protein 1 Antibody, KUB1 Antibody, SGP2 Antibody, APOJ Antibody, SGP-2 Antibody, Sulfated glycoprotein 2 Antibody, CLU2 Antibody, Complement cytolysis inhibitor Antibody, NA1/NA2 Antibody, SP-40 Antibody


Specifications

Target
Human CLU / Clusterin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1A11]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CLU / Clusterin antibody was raised against recombinant human Clusterin (1-333aa) purified from E. coli.
Epitope
aa1-333
Usage
Immunohistochemistry: LS-B2937 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2937 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Immunohistochemistry

Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Western blot

Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Immunohistochemistry

Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Immunohistochemistry

Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Western blot

Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Immunohistochemistry

Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Immunohistochemistry

Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Western blot

Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Immunohistochemistry

Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Immunohistochemistry

Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.

Western blot

Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Requested From: 
Date Requested: 7/18/2018

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