Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1A11] (IgG1,k) to Human CLU / Clusterin
IHC - Paraffin, Western blot, ELISA
Human CLU / Clusterin
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1A11]
Protein G purified
IHC - Paraffin (10 µg/ml)
Western blot (1:1000 - 1:2000)
Specificity and Use
CLU / Clusterin antibody was raised against recombinant human Clusterin (1-333aa) purified from E. coli.
Immunohistochemistry: LS-B2937 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2937 was determined to be 10 ug/ml.
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself.
Anti-Clusterin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Anti-Clusterin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2937 concentration 10 ug/ml.
Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.