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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NCLN Antibody (aa249‑405, clone 9C8G10) LS‑C191813
NCLN antibody LS-C191813 is an unconjugated mouse monoclonal antibody to human NCLN. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C191813-100
100 µg (1 mg/ml)
Unavailable

Popular NCLN Products

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IHC of paraffin-embedded endometrial cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
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Applications: IHC, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

NCLN antibody LS-C191813 is an unconjugated mouse monoclonal antibody to human NCLN. Validated for ELISA, IF, IHC and WB.
About NCLN
May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning. Q969V3 BC003076 NP_064555.2

NCLN Antibody, Nicalin Antibody, Nicalin homolog (zebrafish) Antibody, Nicastrin-like protein Antibody, NET59 Antibody, Nicalin homolog (zebrefish) Antibody


Specifications

Target
Human NCLN
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [9C8G10]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence (1:150 - 1:500)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NCLN antibody was raised against purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli.
Epitope
aa249-405
Specificity
Human
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.
Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.

Western blot

Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)

Western blot

Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.
Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.

Western blot

Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)

Western blot

Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.
Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.

Western blot

Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)

Western blot

Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using CLGN mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HepG2 cells using CLGN mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.
Western blot using CLGN mouse monoclonal antibody against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.

Western blot

Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Western blot using CLGN monoclonal antibody against human CLGN recombinant protein. (Expected MW is 43.5 kDa)

Western blot

Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CLGN monoclonal antibody against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/20/2018

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