Mouse CLEC7A / Dectin 1

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

2A11

Unconjugated. Also available conjugated with RPE, Biotin, FITC.

Protein G purified

Unmodified. Also available Low Endotoxin.

Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.

Recognizes murine beta-glucan receptor, also known as Dectin-1. Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macrophages via the protein tyrosine kinase known as Syk. Inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version is recommended for functional studies.

• IHC - Frozen
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:50)

• IHC - Paraffin
• IHC - Resin

The epitope recognized by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation should be used for frozen sections.

PBS, 0.09% Sodium Azide

Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BXN2 NCBI: NM_022570 NP_072092.2