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Anti-CLDN7 / Claudin 7 Antibody (C-Terminus) IHC-plus™ LS-B2918

Note: This antibody replaces LS-C6162

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

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Most Popular CLDN7 / Claudin 7 Antibodies

Anti-CLDN7 / Claudin 7 Antibody (C-Terminus) IHC-plus™ LS-B2918
Mouse Monoclonal (IgG2a,k) to Human CLDN7 / Claudin 7
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

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Mouse Monoclonal (IgG2a,k) to Human CLDN7 / Claudin 7
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CLDN7 / Claudin 7
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

CLDN7 / Claudin 7 antibody was raised against synthetic peptide derived from the C-terminal region of the human Claudin-7 protein.
C-Terminus
Specific for the claudin-7 protein and does not cross-react with Claudin-3 or -4. On Western blots, it identifies a band at ~22kD. Reactivity has been confirmed with T47D cells by Western blotting and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry.
Immunohistochemistry: LS-B2918 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2918 was determined to be 10 ug/ml. Positive control: Western Blot: T47D cells.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CLDN7 / Claudin 7

O95471 NM_001307 NP_001298.3

CLDN7 Antibody, Claudin-7 Antibody, Claudin-1 Antibody, CLDN-7 Antibody, Hs.84359 Antibody, CEPTRL2 Antibody, Claudin 7 Antibody, CPETRL2 Antibody

CLDN7 / Claudin 7 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.

Immunohistochemistry

Immunohistochemistry
Anti-CLDN7 / Claudin-7 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2918 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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