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Anti-CLDN7 / Claudin 7 Antibody (C-Terminus) IHC-plus™ LS-B2918

Note: This antibody replaces LS-C6162

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular CLDN7 / Claudin 7 Antibodies

Anti-CLDN7 / Claudin 7 Antibody (aa107-120) IHC-plus™ LS-B798
Rabbit Polyclonal to Human CLDN7 / Claudin 7
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLDN7 / Claudin 7 Antibody (C-Terminus) IHC-plus™ LS-B2918
Mouse Monoclonal (IgG2a,k) to Human CLDN7 / Claudin 7
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLDN7 / Claudin 7 Antibody (C-Terminus) IHC-plus™ LS-B5188
Rabbit Polyclonal (IgG) to Human CLDN7 / Claudin 7
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a,k) to Human CLDN7 / Claudin 7
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human CLDN7 / Claudin 7
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CLDN7 / Claudin 7 antibody was raised against synthetic peptide derived from the C-terminal region of the human Claudin-7 protein.
C-Terminus
Specific for the claudin-7 protein and does not cross-react with Claudin-3 or -4. On Western blots, it identifies a band at ~22kD. Reactivity has been confirmed with T47D cells by Western blotting and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry.
Immunohistochemistry: LS-B2918 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2918 was determined to be 10 ug/ml. Positive control: Western Blot: T47D cells.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CLDN7 / Claudin 7

O95471 NM_001307 NP_001298.3

CLDN7 Antibody, Claudin-7 Antibody, Claudin-1 Antibody, CLDN-7 Antibody, Hs.84359 Antibody, CEPTRL2 Antibody, Claudin 7 Antibody, CPETRL2 Antibody

CLDN7 / Claudin 7 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.

Immunohistochemistry

Immunohistochemistry
Anti-CLDN7 / Claudin-7 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2918 concentration 10 ug/ml.
 

Requested From: 
Date Requested: 3/26/2017

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