Human CLDN4 / Claudin 4

Mouse

Human, Rat, Dog (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

3E2C1

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-Terminus of human Claudin 4

C-Terminus

Recognizes the human ~22kD Claudin 4 protein and does not cross-react with the protein at 55kD. Species cross-reactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry.

• IHC - Paraffin (5 µg/ml)
• Immunofluorescence (1 - 3 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B2257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2257 was determined to be 5 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O14493 NCBI: NM_001305 NP_001296.1