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Anti-CLCF1 Antibody IHC-plus™ LS-B8220

Note: This antibody replaces LS-C104582

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CLCF1 Antibodies

Anti-CLCF1 Antibody IHC-plus™ LS-B8220
Rabbit Polyclonal to Human CLCF1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLCF1 Antibody (aa28-225, Biotin) LS-C297929
Rabbit Polyclonal (IgG) to Mouse CLCF1
Mouse
Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-CLCF1 Antibody (aa28-225, FITC) LS-C304843
Rabbit Polyclonal (IgG) to Mouse CLCF1
Mouse
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CLCF1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CLCF1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)

Specificity and Use

CLCF1 antibody was raised against e.coli derived recombinant Human NNT-1/BCSF-3.
Human NNT-1/BCSF-3
Immunohistochemistry: LS-B8220 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NNT-1/BCSF-3 (LS-C104491) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CLCF1

Q9UBD9 NM_013246 NP_037378.1

CLCF1 Antibody, B-cell stimulating factor 3 Antibody, BSF-3 Antibody, BSF3 Antibody, CISS2 Antibody, NR6 Antibody, Novel neurotrophin-1 Antibody, NNT1 Antibody, B-cell stimulatory factor 3 Antibody, B-cell-stimulating factor 3 Antibody, NNT-1 Antibody

CLCF1 is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR.

Immunohistochemistry

Immunohistochemistry
Anti-CLCF1 antibody IHC of human lung, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8220 dilution 5 ug/ml.
 

Requested From: United States
Date Requested: 12/4/2016

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