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Anti-CLCF1 Antibody (Biotin) LS-C104491

Note: This antibody replaces LS-C37805

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CLCF1 Antibodies

Anti-CLCF1 Antibody IHC-plus™ LS-B8220
Rabbit Polyclonal to Human CLCF1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLCF1 Antibody (aa28-225, Biotin) LS-C297929
Rabbit Polyclonal (IgG) to Mouse CLCF1
Mouse
Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-CLCF1 Antibody (aa28-225, FITC) LS-C304843
Rabbit Polyclonal (IgG) to Mouse CLCF1
Mouse
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CLCF1
Human
Western blot, ELISA
Biotin Conjugated

Details

Human CLCF1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

CLCF1 antibody was raised against e.coli derived recombinant Human NNT-1/BCSF-3.
Human NNT-1 /BCSF-3
ELISA: To detect hNNT-1/BCSF-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hNNT-1/BCSF-3. To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human NNT-1/BCSF-3 (LS-B8220) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CLCF1

Q9UBD9 NM_013246 NP_037378.1

CLCF1 Antibody, B-cell stimulating factor 3 Antibody, BSF-3 Antibody, BSF3 Antibody, CISS2 Antibody, NR6 Antibody, Novel neurotrophin-1 Antibody, NNT1 Antibody, B-cell stimulatory factor 3 Antibody, B-cell-stimulating factor 3 Antibody, NNT-1 Antibody

CLCF1 is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR.

Requested From: United States
Date Requested: 12/10/2016

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