Human CKS1B / CKS1

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of human and mouse Cks1.

C-Terminus

Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.

• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunoprecipitation: 7 ug/IP reaction. Immunohistochemistry: 2.5 ug/ml. Note: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epitope Retrieval (HIER) with citrate buffer, pH 6.0, is recommended.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61024 NCBI: NM_001826 NP_001817.1