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Anti-CKMT1B Antibody (clone 2C8) LS-C196925

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CKMT1B Antibodies

Anti-CKMT1B Antibody LS-C311056
Rabbit Polyclonal (IgG) to Human CKMT1B
Human
Immunoprecipitation
Unconjugated
Anti-CKMT1B Antibody LS-C408648
Rabbit Polyclonal (IgG) to Human CKMT1B
Human, Rat
IHC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2C8] (IgG2a,k) to Human CKMT1B
Human
Western blot, ELISA, RNA interference
Unconjugated

Details

Human CKMT1B
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2C8]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

CKMT1B antibody was raised against cKMT1B (NP_066270, aa327-417 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CKMT1B
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CKMT1B

P12532 NM_020990 NP_066270.1

CKMT1B Antibody, CKMT1 Antibody, Mia-CK Antibody, Mt-ck Antibody, Ubiquitous MtCK Antibody, U-MtCK Antibody, CKMT Antibody, Mtck Antibody, UMTCK Antibody

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.

Western blot

Western blot
Western Blot analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody (M04), clone 2C8.Lane 1: CKMT1B transfected lysate(47 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
CKMT1B monoclonal antibody (M04), clone 2C8. Western Blot analysis of CKMT1B expression in A-431.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CKMT1B is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CKMT1B monoclonal antibody (M04), clone 2C8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: United States
Date Requested: 12/9/2016

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