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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CKM / Creatine Kinase MM Antibody (aa283‑313) LS‑C99019
Creatine Kinase MM antibody LS-C99019 is an unconjugated rabbit polyclonal antibody to human Creatine Kinase MM (CKM). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C99019-400
400 µl
Unavailable

Popular CKM / Creatine Kinase MM Products

Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: ELISA
Anti-CKM / Creatine Kinase MM antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Western blot of recombinant CKM / Creatine Kinase MM.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Pig
Applications: IHC, Western blot
Anti-CKM / Creatine Kinase MM antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11194 concentration 5 ug/ml.
Species: Human, Mouse, Dog, Hamster, Horse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Creatine Kinase MM antibody LS-C99019 is an unconjugated rabbit polyclonal antibody to human Creatine Kinase MM (CKM). Validated for Flow, IHC and WB.
About CKM / Creatine Kinase MM
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. P06732 NM_001824 NP_001815.2

CKM Antibody, CK-MB Antibody, CKMM Antibody, Creatine kinase M-type Antibody, Creatine kinase, muscle Antibody, M-CK Antibody, Creatine kinase M chain Antibody


Specifications

Target
Human CKM / Creatine Kinase MM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Pig, Rabbit, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8662 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99019. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa283-313
Specificity
This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.
The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.

Flow Cytometry

Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.
The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.

Flow Cytometry

Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.
The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.

Flow Cytometry

Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.
The anti-CKM antibody is used in Western blot to detect CKM in C6 cell lysate.

Flow Cytometry

Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of HepG2 cells using Creatine Kinase MB (CKM) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/18/2018

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