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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CIITA Antibody (N‑Terminus) IHC‑plus™ LS‑B5490
Note: This antibody replaces LS-C115907
CIITA antibody LS-B5490 is an unconjugated rabbit polyclonal antibody to CIITA from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5490-50
50 µg (1 mg/ml)
Unavailable

Popular CIITA Products

Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA. In panel A, both samples on PVDF were incubated with 10 mg/mL mouse anti-FLAG antibody (Sigma) for 45'. In panel B, both samples on PVDF were incubated with a 1:500 dilution of anti-CIITA (1-333) for 45'.
Species: Human
Applications: Western blot, ELISA
NIH 3T3 Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Dog
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Western blot of extracts from K562 cells, using CIITA Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CIITA antibody LS-B5490 is an unconjugated rabbit polyclonal antibody to CIITA from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CIITA
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. P33076 NM_000246 NP_000237.2

CIITA Antibody, C2TA Antibody, MHC class II transactivator Antibody, MHC2TA Antibody, CIITAIV Antibody, NLRA Antibody


Specifications

Target
Human CIITA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E31001 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B5490. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
CIITA antibody can be used for detection of CIITA by Western blot at 1-2 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.
Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.

Western blot

Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.
Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.

Western blot

Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.
Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.

Immunohistochemistry

Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.
Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.

Western blot

Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.
Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.

Western blot

Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.
Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.

Immunohistochemistry

Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.
Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.

Western blot

Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.
Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.

Western blot

Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.
Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.

Immunohistochemistry

Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.
Anti-CIITA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5490 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.
Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 ug/ml.

Western blot

Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.
Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 µg/mL.

Western blot

Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.
Western blot analysis of CIITA in mouse brain tissue lysate with CIITA antibody at 1 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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