Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4E1] (IgG3) to Human Chymotrypsin
Human, Mouse, Rat
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG3 Monoclonal [4E1]
Protein A purified
IHC - Paraffin (5 - 10 µg/ml)
IHC - Frozen
Specificity and Use
Chymotrypsin antibody was raised against purified human pancreatic chymotrypsin.
Human chymotrypsin-C, also known as chymotrypsin or caldecrin. Chymotrypsin is a 239 amino acid ~30 kD protease with an additional 13 amino acid propeptide region and a 16 amino acid signal peptide. Variations in the CTRC gene has been associated with susceptibility to hereditary, pancreatitis, a disease characterized by pancreatic inflammation and destruction of the parenchyma.
PBS, pH 7.2, 0.09% sodium azide. Sourced in Ascites.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
Chymotrypsin is a digestive enzyme component of pancreatic juice acting in the duodenum where it performs proteolysis, the breakdown of proteins and polypeptides. Chymotrypsin preferentially cleaves peptide amide bonds where the carboxyl side of the amide bond (the P1 position) is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine). These amino acids contain an aromatic ring in their sidechain that fits into a 'hydrophobic pocket' (the S1 position) of the enzyme.