Target
Human CHUK / IKKA / IKK Alpha
Synonyms
CHUK | I-kappa-B kinase alpha | I-kappa-B kinase-alpha | Ikb kinase alpha | IKK-A | IKBKA | IKK1 | NFKBIKA | I-kappa-B kinase 1 | IkappaB kinase | IKK Alpha | IKK-alpha | IKKA | Transcription factor 16 | IkappaBalpha Kinase | IkB kinase alpha subunit | IKK-a kinase | Ikkalpha | TCF-16 | TCF16
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Synthetic phosphopeptide derived from the region of human IKKa that contains serine 176 and serine 180. The sequence is conserved in mouse.
Specificity
Recognizes human IKKa. Species sequence homology: mouse-100%. Sequence homology: IKKb-93%.
Applications
- Western blot (0.5 - 1.5 µg/ml)
Usage
Suitable for use in Western Blot. Western Blot: 0.5-1.5 ug/ml. at 0.75 ug/ml, the dilution provides 100 ml working solution, which 10 ml/blot allows 10 blots to be performed. When examining phosphorylation of endogenous IKKa protein, we recommend that IKKa first be immunoprecipitated. Positive control: Immunoprecipitates prepared from lysates of Jurkat cells, treated with TNF-a.
Presentation
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, before tha addition of glycerol to 40%.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHUK / IKKA / IKK Alpha