Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey (tested or 100% immunogen sequence identity)
Western blot (1 - 3 µg/ml)
Specificity and Use
CHUK / IKKA / IKK Alpha antibody was raised against synthetic peptide EHDHSLSCVVTPQD from an internal region (near C-terminus) of human CHUK / IKKA / IKK Alpha (NP_001269.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Mouse, Rat (64%).
Human CHUK / IKKA / IKK Alpha
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80kD band observed in nuclear lysates of cell line HeLa (calculated MW of 84.6kD according to NP_001269.3). Recommended concentration: 1-3 ug/ml.
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome.