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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHTF18 / RUVBL Antibody (aa755‑804) LS‑C116698
RUVBL antibody LS-C116698 is an unconjugated rabbit polyclonal antibody to RUVBL (CHTF18) from human, mouse, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C116698-50
50 µg
Unavailable

Popular CHTF18 / RUVBL Products

Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, Dot Blot
Detection of Human CTF18 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CTF18 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human CTF18 by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CTF18 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Western blot analysis of immunized recombinant protein, using anti-CHTF18 monoclonal antibody.
Species: Human
Applications: Western blot, Immunoprecipitation, Dot Blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

RUVBL antibody LS-C116698 is an unconjugated rabbit polyclonal antibody to RUVBL (CHTF18) from human, mouse, dog and other species. Validated for WB.
About CHTF18 / RUVBL
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). Q8WVB6 Q8WVB6

CHTF18 Antibody, C16orf41 Antibody, C321D2.2 Antibody, C321D2.4 Antibody, C321D2.3 (novel protein) Antibody, Ctf18 Antibody, HCTF18 Antibody, Homolog of yeast CHL12 Antibody, C321D2.3 Antibody, C321D2.4 (novel protein) Antibody, CHL12 Antibody, RUVBL Antibody


Specifications

Target
Human CHTF18 / RUVBL
Host
Rabbit
Reactivity
Human, Mouse, Dog, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Bat, Bovine, Guinea pig, Horse, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CHTF18 / RUVBL antibody was raised against synthetic peptide located between aa755-804 of human CHTF18 (Q8WVB6, NP_071375). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Panda, Dog, Opossum (100%); Marmoset, Rat, Elephant, Bovine, Bat, Horse, Guinea pig, Turkey, Chicken (92%); Platypus, Xenopus, Zebrafish (83%).
Blocking Peptide
LS-E21025 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116698. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa755-804
Specificity
Human CHTF18
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHTF18 antibody LS-C116698 Western blot of Fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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