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Catalog Number	Size	Price
LS-C112799-100	100 µg (0.5 mg/ml)	$503

CHRNA4 / NACHR Antibody - CHRNA4 antibody (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Human CHRNA4 / NACHR Antibody (aa29‑43, WB) LS‑C112799

Name: Anti-CHRNA4 / NACHR Antibody | Goat anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C112799-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

CHRNA4 / NACHR Goat anti-Human Polyclonal (aa29-43) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Rat, Chicken

Format:

Unconjugated, Unmodified

$503

LS-C112799-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CHRNA4 / NACHR Goat anti-Human Polyclonal (aa29-43) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Rat, Chicken

Format:

Unconjugated, Unmodified

Specifications

Description

NACHR antibody LS-C112799 is an unconjugated goat polyclonal antibody to NACHR (CHRNA4) (aa29-43) from human. It is reactive with human, rat, chicken and other species. Validated for Peptide-ELISA and WB.

Target

Human CHRNA4 / NACHR

Synonyms

See All CHRNA4 / NACHR Antibodies

Host

Goat

Reactivity

Human, Monkey, Rat, Chicken (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

Epitope

aa29-43

Specificity

Human CHRNA4. This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Applications

Western blot (1 - 3 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Usage

Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CHRNA4 / NACHR

Uniprot: P43681 NCBI: NM_000744 NP_000735.1