Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC (50 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CHRM4 / M4 antibody was raised against peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein.
Shown to be specific for the COOH terminal of the M4 receptor. Cross-reactivity: M4 Muscarinic Receptor (aa469-478): 100%, M4 Muscarinic Receptor: ~70%. No reactivity is observed with M1-M3, M5 (full receptors), M1 (aa451-460), M2 (aa457-466), M3 Muscarinic Receptor (aa580-589), M5 (aa519-531).
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Immunohistochemistry: 50 ug/ml stains PLP fixed cultured cells known to express the Muscarinic Receptor M4.
PBS, pH 7.2, 10 mg/ml BSA, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.