M4 antibody LS-C9139 is an unconjugated rabbit polyclonal antibody to human M4 (CHRM4). Validated for ELISA, IHC and WB.
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
- IHC (50 µg/ml)
- Western blot
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CHRM4 / M4 antibody was raised against peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein.
Shown to be specific for the COOH terminal of the M4 receptor. Cross-reactivity: M4 Muscarinic Receptor (aa469-478): 100%, M4 Muscarinic Receptor: ~70%. No reactivity is observed with M1-M3, M5 (full receptors), M1 (aa451-460), M2 (aa457-466), M3 Muscarinic Receptor (aa580-589), M5 (aa519-531).
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Immunohistochemistry: 50 ug/ml stains PLP fixed cultured cells known to express the Muscarinic Receptor M4.
PBS, pH 7.2, 10 mg/ml BSA, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.