(tested or 100% immunogen sequence identity)
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CHRM3 / M3 antibody was raised against a peptide analogue of the carboxyl terminal of the M3 receptor covalently attached to a carrier protein.
Recognizes rat M3 Muscarinic Receptor (aa580-589): 100%; M3 Muscarinic Receptor 70%; M1 Muscarinic Receptor (aa451-460) 0%; M1 Muscarinic Receptor 0%; M2 Muscarinic Receptor (aa457-466) 0%; M2 Muscarinic Receptor 0%; M4 Muscarinic Receptor (aa469-478) 0%; M4 Muscarinic Receptor 0%; M5 Muscarinic Receptor (aa419-531) 0%; M5 Muscarinic Receptor 0%. This antibody is also specific for the COOH terminal of the M3 receptor as characterized by Western Blot, ELISA and IHC.
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Immunohistochemistry: 10 ug/ml had been found to stain rat brain sections that express the muscarinic M3 receptor after fixing with PLP fixative and using ABC techniques. Western Blot: homogenates whole rat brain.
PBS, 10 mg/ml BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.