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Antibodies > CHRM1 / M1 > LS-C313301
 
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Catalog Number Size Price
LS-C313301-10 10 µg $318 
LS-C313301-100 100 µg (0.5 mg/ml) $470 
CHRM1 / M1 Antibody - IHC analysis of CHRM1 using anti-CHRM1 antibody. CHRM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHRM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CHRM1 / M1 Antibody - CHRM1 / M1 antibody. IHC(P): Rat Brain Tissue.
CHRM1 / M1 Antibody - IF analysis of CHRM1 using anti-CHRM1 antibody CHRM1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CHRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CHRM1 / M1 Antibody - IF analysis of CHRM1 using anti-CHRM1 antibody CHRM1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CHRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CHRM1 / M1 Antibody - Western blot analysis of CHRM1 using anti-CHRM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U87 whole cell lysates, Lane 2: SHG whole cell lysates, Lane 3: NEURO whole cell lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHRM1 at approximately 51KD. The expected band size for CHRM1 is at 51KD.
CHRM1 / M1 Antibody - WB of CHRM1 / M1 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Mouse Brain Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: SHG Cell Lysate. Lane 5: NEURO Cell Lysate. Lane 6: HELA Cell Lysate.
CHRM1 / M1 Antibody - IHC analysis of CHRM1 using anti-CHRM1 antibody. CHRM1 was detected in frozen section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHRM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CHRM1 / M1 Antibody - CHRM1 / M1 antibody. IHC(P): Rat Brain Tissue.
CHRM1 / M1 Antibody - IF analysis of CHRM1 using anti-CHRM1 antibody CHRM1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CHRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CHRM1 / M1 Antibody - IF analysis of CHRM1 using anti-CHRM1 antibody CHRM1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CHRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CHRM1 / M1 Antibody - Western blot analysis of CHRM1 using anti-CHRM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U87 whole cell lysates, Lane 2: SHG whole cell lysates, Lane 3: NEURO whole cell lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHRM1 at approximately 51KD. The expected band size for CHRM1 is at 51KD.
CHRM1 / M1 Antibody - WB of CHRM1 / M1 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Mouse Brain Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: SHG Cell Lysate. Lane 5: NEURO Cell Lysate. Lane 6: HELA Cell Lysate.
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Polyclonal Rabbit anti‑Human CHRM1 / M1 Antibody (aa303‑317, IHC, WB) LS‑C313301

Polyclonal Rabbit anti‑Human CHRM1 / M1 Antibody (aa303‑317, IHC, WB) LS‑C313301

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Note: This antibody replaces LS-C387932
Antibody:
CHRM1 / M1 Rabbit anti-Human Polyclonal (aa303-317) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313301-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CHRM1 / M1 Rabbit anti-Human Polyclonal (aa303-317) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
M1 antibody LS-C313301 is an unconjugated rabbit polyclonal antibody to M1 (CHRM1) (aa303-317) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CHRM1 / M1
Synonyms
CHRM1 | Acetylcholine receptor M1 | M1R | M1 | M1 muscarinic receptor | HM1 | Muscarinic M1 receptor
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human muscarinic Acetylcholine Receptor 1(303-317 aa KMPMVDPEAQAPTKQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Epitope
aa303-317
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHRM1 / M1
P11229 NM_000738 NP_000729.2

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Featured Products

Product
CHRM1 / M1 Antibody (C‑Terminus) LS‑C37391
Species: Human
Applications: IHC, Western blot, ELISA
CHRM1 / M1 Antibody - Goat Anti-M1 mAChR / CHRM1 Antibody (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
CHRM1 / M1 Antibody (aa438‑453) LS‑C54654
Species: Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CHRM1 / M1 Antibody - Western blot analysis of lysates from HepG2 cells, using CHRM1 Antibody. The lane on the right is blocked with the synthesized peptide.
CHRM1 / M1 Antibody (aa295‑344) LS‑C120626
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CHRM1 / M1 Antibody - Western blot analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
CHRM1 / M1 Antibody LS‑C331571
Species: Human, Mouse, Rat
Applications: IHC, Western blot
CHRM1 / M1 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CHRM1 / M1 Antibody (270‑350 aa, Internal) LS‑B15692
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
TNFSF13 / APRIL Antibody - Anti-APRIL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ TNFSF13 / APRIL Antibody (aa151‑200) LS‑B7666
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CHRM1 / M1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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