Target
Human Chondroitin 6 Sulfate
Reactivity
Human, Mouse, Rat, Rabbit, Sheep
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Chondroitinase ABC-digested adult human aggrecan.
Specificity
Recognizes human Chondroitin 6 Sulfate. Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. Does not react with native proteoglycans, dermatan sulfate, keratan sulfate or chondroitin-4-sulfate. Species cross-reactivity: chondroitin-6-sulfate stubs of all proteoglycans of all species.
Applications
- ELISA (1:100 - 1:5000)
- Radioimmunoassay (1:100 - 1:200)
Usage
Suitable for use in ELISA and RIA. ELISA: 1:100-1:5000. RIA: 1:100-1:200. Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (e. g. with papain or pronase) significantly reduces the antibody reactivity.
Presentation
No preservatives added
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee