Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Vibrio cholerae Cholera Toxin
Western blot, ELISA
Vibrio cholerae Cholera Toxin
Vibrio cholerae (tested or 100% immunogen sequence identity)
Western blot (1:20 - 1:200)
ELISA (1:200 - 1:1000)
Specificity and Use
Cholera Toxin antibody was raised against purified Choleragenoid.
Recognizes the beta subunit of cholera toxin. The beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin alpha subunit into cells. The holotoxin consists of a pentameric ring of beta subunits whose central pore is occupied by the alpha subunit. The alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The alpha subunit (and cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 0.002% Thimerosal, 1% BSA.
+4°C or -20°C, Avoid repeated freezing and thawing.