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Anti-CHGA / Chromogranin A Antibody (clone LK2H10) IHC-plus™ LS-B5571

Note: This antibody replaces LS-C134865

Ordering

Wt. Vol. Conc. Price
- 50 µl - Unavailable

Most Popular CHGA / Chromogranin A Antibodies

Anti-CHGA / Chromogranin A Antibody (C-Terminus) IHC-plus™ LS-B1515
Rabbit Polyclonal (IgG) to Human CHGA / Chromogranin A
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CHGA / Chromogranin A Antibody (aa85-97) IHC-plus™ LS-B4331
Goat Polyclonal to Human CHGA / Chromogranin A
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CHGA / Chromogranin A Antibody (clone LK2H10) IHC-plus™ LS-B5571
Mouse Monoclonal [clone LK2H10] (IgG1) to Human CHGA / Chromogranin A
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone LK2H10] (IgG1) to Human CHGA / Chromogranin A
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated

Details

Human CHGA / Chromogranin A
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [LK2H10]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot

Specificity and Use

The antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kD protein. Antigen was isolated from human Pheochromocytoma. Antigen location, cytoplasm. Cross-reactivity with monkey and pig.
This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody. Recommended for positive control: Adrenal medulla, pancreas. The antibody can be used on acetone fixed frozen sections and formalin fixed paraffin embedded tissue. Pretreatment is not necessary. Optimal dilution should be tested by serial dilutions, 1:10 is advised based on the Indirect method. With a normal ABC method the dilution can be 5-10 times higher. Dilute immediately before use with PBS or Tris.

Packaging

Ascites, Tris/HCl buffer, 0.09% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CHGA / Chromogranin A

P10645 NM_001275 NP_001266.1

CHGA Antibody, Chromogranin A Antibody, Chromogranin-A Antibody, Pancreastatin Antibody, Pituitary secretory protein I Antibody, Vasostatin I Antibody, SP-I Antibody, Vasostatin II Antibody

CHGA / Chromogranin A is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.

Immunohistochemistry

Immunohistochemistry
Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5571 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-CHGA / Chromogranin A antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5571 dilution 1:100.

Requested From: 
Date Requested: 3/27/2017

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