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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHFR Antibody (clone 1H3‑A12) LS‑C133778
CHFR antibody LS-C133778 is an unconjugated mouse monoclonal antibody to human CHFR. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C133778-100
100 µg
Unavailable

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Product Description

CHFR antibody LS-C133778 is an unconjugated mouse monoclonal antibody to human CHFR. Validated for ELISA, IHC and WB.
About CHFR
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. NM_018223

CHFR Antibody, RING finger protein 196 Antibody, RNF196 Antibody, RNF116 Antibody


Specifications

Target
Human CHFR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1H3-A12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHFR antibody was raised against cHFR (AAH12072, 1 a.a. ~ 653 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CHFR
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.
Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.
Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.
Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.
Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.


Requested From: 
Date Requested: 6/20/2018

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