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Anti-CHFR Antibody (clone 1H3-A12) LS-C133778


Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CHFR Antibodies

Anti-CHFR Antibody (aa36-85) IHC-plus™ LS-B5739
Rabbit Polyclonal (IgG) to Human CHFR
Human, Monkey, Mouse, Rat, Guinea pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-CHFR Antibody (aa476-504, AP) LS-C254726
Rabbit Polyclonal (IgG) to Human CHFR
Western blot, ELISA
AP Conjugated
Anti-CHFR Antibody (aa476-504, FITC) LS-C254729
Rabbit Polyclonal (IgG) to Human CHFR
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1H3-A12] (IgG1,k) to Human CHFR
IHC - Paraffin, Western blot, ELISA


Human CHFR
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1H3-A12]
Protein A purified


  • IHC - Paraffin (2 µg/ml)
  • Western blot

Specificity and Use

CHFR antibody was raised against cHFR (AAH12072, 1 a.a. ~ 653 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CHFR
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.


PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CHFR


CHFR Antibody, RING finger protein 196 Antibody, RNF196 Antibody, RNF116 Antibody

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome.


Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody, clone 1H3-A12. GAPDH ( 36.1 kD ) used as specificity and loading control.

Requested From: United States
Date Requested: 10/22/2016

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