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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHEK2 / CHK2 Antibody (clone 73C175.1.1) IHC‑plus™ LS‑B1321
Note: This antibody replaces LS-C508
CHK2 antibody LS-B1321 is an unconjugated mouse monoclonal antibody to human CHK2 (CHEK2). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1321-50
50 µg
Unavailable

Popular CHEK2 / CHK2 Products

Antibody
Species: Human
Applications: Western blot
Anti-CHEK2 / CHK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1348 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, Western blot
Anti-CHEK2 / CHK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6212 concentration 3 ug/ml.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
IHC of p-Chk2 (T68) pAb in paraffin-embedded human breast carcinoma tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CHK2 antibody LS-B1321 is an unconjugated mouse monoclonal antibody to human CHK2 (CHEK2). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CHEK2 / CHK2
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. O96017 NM_007194 NP_009125.1

CHEK2 Antibody, BA444G7 Antibody, Cds1 homolog Antibody, Checkpoint kinase 2 Antibody, Checkpoint-like protein CHK2 Antibody, CHK2 checkpoint homolog Antibody, CHK2 Antibody, HCds1 Antibody, LFS2 Antibody, RAD53 Antibody, Rad53 homolog Antibody, HCHK2 Antibody, HuCds1 Antibody, PP1425 Antibody


Specifications

Target
Human CHEK2 / CHK2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [73C175.1.1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHEK2 / CHK2 antibody was raised against a synthetic peptide obtained from human Chk2 protein sequence.
Specificity
A synthetic peptide obtained from human Chk2 protein sequence was used as immunogen.
Usage
Immunohistochemistry: LS-B1321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1321 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.

Western blot

Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

Immunohistochemistry

Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.

Western blot

Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

Immunohistochemistry

Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.

Western blot

Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

Immunohistochemistry

Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.

Western blot

Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

Requested From: 
Date Requested: 7/15/2018

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