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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHEK1 / CHK1 Antibody (Lys274) LS‑C175954
CHK1 antibody LS-C175954 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1). Validated for WB.
Catalog
Size
Price
LS-C175954-100
100 µl
Unavailable

Popular CHEK1 / CHK1 Products

Anti-Chk1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B932 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, Western blot
Detection of human Chk1 in whole cell lysates of HeLa cells by immunoprecipitation and Western blotting using Goat anti-Human Chk1(LS-C43054). Immunoprecipitated material was detected in Western blotting with a monoclonal anti-Chk1 antibody.
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Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

CHK1 antibody LS-C175954 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1). Validated for WB.
About CHEK1 / CHK1
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. O14757 NM_001274 NP_001265.2

CHEK1 Antibody, CHK1 checkpoint homolog Antibody, Cell cycle checkpoint kinase Antibody, Checkpoint kinase 1 Antibody, CHK1 Antibody, Chk1-S Antibody, Protein kinase chk1 Antibody, Checkpoint kinase-1 Antibody


Specifications

Target
Human CHEK1 / CHK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
Immunogen
CHEK1 / CHK1 antibody was raised against synthetic peptide around Lys274 of human CHK1
Epitope
Lys274
Specificity
Human CHK1
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Western blot

Western blot of Chk1 (K274) pAb in extracts from HeLa cells.
Western blot of Chk1 (K274) pAb in extracts from HeLa cells.

Western blot

Western blot of Chk1 (K274) pAb in extracts from HeLa cells.
Western blot of Chk1 (K274) pAb in extracts from HeLa cells.

Western blot

Western blot of Chk1 (K274) pAb in extracts from HeLa cells.
Western blot of Chk1 (K274) pAb in extracts from HeLa cells.

Western blot

Western blot of Chk1 (K274) pAb in extracts from HeLa cells.
Western blot of Chk1 (K274) pAb in extracts from HeLa cells.


Requested From: 
Date Requested: 6/25/2018

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