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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60087-100 | 100 µg (0.73 mg/ml) | $567 |
![CHEK1 / CHK1 Antibody - Anti-Yeast CHK1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast CHK1 antibody shows detection of a bands corresponding to CHK1 in Saccharomyces cerevisiae lysates. Two strains of S. cerevisiae were tested. Lane 1 shows a predominant band at ~60 kD. Lane 2 shows a predominant band at ~38 kD. Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively). Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/chek1-chk1-antibody-ls-c60087/62922_192391.jpg)
Polyclonal Rabbit anti‑Saccharomyces cerevisiae CHEK1 / CHK1 Antibody (WB) LS‑C60087
Polyclonal Rabbit anti‑Saccharomyces cerevisiae CHEK1 / CHK1 Antibody (WB) LS‑C60087
Antibody:
CHEK1 / CHK1 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae, Yeast
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CHEK1 / CHK1 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae, Yeast
Format:
Unconjugated, Unmodified
Specifications
Description
CHK1 antibody LS-C60087 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) from saccharomyces cerevisiae. It is reactive with saccharomyces cerevisiae and yeast. Validated for ELISA, IP and WB.
Target
Saccharomyces cerevisiae CHEK1 / CHK1
Synonyms
CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
Host
Rabbit
Reactivity
Saccharomyces cerevisiae, Yeast
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.
Specificity
A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.
Applications
- Western blot (1:200 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:2000 - 1:10000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kD in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CHEK1 / CHK1
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Dot Blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
![CHEK1 / CHK1 Antibody - Immunofluorescence of monoclonal antibody to CHEK1 on HeLa cell . [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/chek1-chk1-antibody-clone-2g3-ls-c196906/144310_498563.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
