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CHAF1B / CAF1

chromatin assembly factor 1, subunit B (p60)

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Name: chromatin assembly factor 1, subunit B (p60)
Synonyms: CHAF1B, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF1A, CAF-1, CAF-IP60, CAF1, CAF1P60, M-phase phosphoprotein 7, MPHOSPH7
Target Sequences: NM_005441 NP_005432.1 Q13112

Publications (5)

1
Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. Smith S, Stillman B. The Journal of biological chemistry. 1991 266:12041-7. [PubMed:2050697]
2
Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Shibahara K, Stillman B. Cell. 1999 96:575-85. [PubMed:10052459]
3
Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. Green CM, Almouzni G. The EMBO journal. 2003 22:5163-74. [PubMed:14517254] [PMC:PMC204462]
4
In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY. Molecular and cellular biology. 2008 28:3672-85. [PubMed:18378699] [PMC:PMC2423286]
5
HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Elsaesser SJ, Allis CD. Cold Spring Harbor symposia on quantitative biology. 2010 75:27-34. [PubMed:21047901]
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Antibodies (36)
Proteins (6)
ELISA Kits (1)
Over-Expression Lysate (2)
Primary (36)
Recombinant (4)
Sandwich (1)
CHAF1B / CAF1 (43)
Human (28)
Mouse (23)
Rat (13)
Bat (3)
Chicken (3)
Chimpanzee (3)
Dog (5)
Gibbon (6)
Guinea pig (3)
Horse (3)
Monkey (5)
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Xenopus (3)
IHC (12)
IHC-P (6)
WB (33)
ELISA (3)
ICC (3)
IF (9)
IP (5)
Peptide-ELISA (3)
rabbit (28)
mouse (8)
IgG (14)
IgG1 (7)
IgG2b,k (1)
monoclonal mc (8)
polyclonal pc (28)
2G7 (1)
OTI2D5 (2)
OTI4F7 (2)
p60-24 (1)
SS 24 1-68 (1)
SS 53 (1)
96-Well Strip Plate (1)
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Biotin Conjugated (3)
Carrier-free (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (23)
aa1-199 (8)
C-Terminus (4)
N-Terminus (4)
aa509-C-term (2)
40-120 aa, Internal (1)
Trp93 (1)
aa375-425 (1)
aa44-93 (1)
aa71-120 (1)
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Wheat Germ Extract (2)
Purified (1)
CHAF1B / CAF1 Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (OTI4F7) Antibody
Mouse, Dog, Rat, Human, Monkey
IF, IHC, IHC-P, WB
Unconjugated
100 µl/$434
CHAF1B / CAF1 Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (Carrier-free) (OTI4F7) Antibody
Mouse, Dog, Rat, Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100 µg/$511
CHAF1B / CAF1 Antibody
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (40-120 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
CHAF1B / CAF1 Antibody - Immunohistochemistry of paraffin-embedded mouse kidney tissue.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CHAF1B / CAF1 Antibody - Western blot of CAF-1 p60 (W93) pAb in extracts from Jurkat cells.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (Trp93) Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
CHAF1B / CAF1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
CHAF1B / CAF1 Antibody - CAF-1 p60 Antibody (SS 24 1-68) -  CAF-1 p60 (SS24 1-68) - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (SS 24 1-68) Antibody
Mouse, Human, Primate
ICC, IP, WB
Unconjugated
0.05 ml/$386
CHAF1B / CAF1 Antibody - Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1 ug/ml).
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (aa509-C-term) Antibody
Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$397
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody
Human, Gibbon
IHC, IHC-P, WB
HRP Conjugated
100 µl/$442
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Human, Gibbon
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$442
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody
Human, Gibbon
IHC, IHC-P, WB
FITC Conjugated
100 µl/$442
CHAF1B / CAF1 Antibody - Anti-CHAF1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (OTI2D5) Antibody
Human
IF, WB
Unconjugated
100 µl/$434
CHAF1B / CAF1 Antibody - Anti-CHAF1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (Carrier-free) (OTI2D5) Antibody
Human
IF, WB
Unconjugated, Carrier-free
100 µg/$511
CHAF1B / CAF1 Antibody - Detection limit for recombinant GST tagged CHAF1B is 0.1 ng/ml as a capture antibody.
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (2G7) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
CHAF1B / CAF1 Antibody
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (p60-24) Antibody
Human
ICC, IP
Unconjugated
100 µg/$392
CHAF1B / CAF1 Antibody - Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
CHAF1B / CAF1 Antibody - CAF-1 p60 (SS53) Antibody - Western Blot of: A. RKO cells with p150 ab, PCNA ab, CHAF1B Antibody CAF-1 p60 antibody B. p150 RNAi knockdown
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CHAF1B / CAF1 Mouse anti-Human Monoclonal (SS 53) Antibody
Human
IP, WB
Unconjugated
0.1 ml/$386
CHAF1B / CAF1 Antibody - Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (aa71-120) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
CHAF1B / CAF1 Antibody - Western blot analysis of the lysates from RAW264.7cells using CAF1B antibody.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (aa44-93) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
CHAF1B / CAF1 Antibody - Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (aa509-C-term) Antibody
Human
IHC
Unconjugated
2.5 µg/$295; 100 µl/$532
CHAF1B / CAF1 Antibody - Western blot of CHAF1B / CAF1 antibody.
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CHAF1B / CAF1 Rabbit anti-Mouse Polyclonal (aa1-199) Antibody
Mouse
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal Antibody
Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CHAF1B / CAF1 Antibody - CHAF1B antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHAF1B / CAF1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
Viewing 1-25 of 43 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).