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Anti-CHAF1A / CAF1 Antibody (clone SS 1 1-13) IHC-plus™ LS-B242

Note: This antibody replaces LS-C2625

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Most Popular CHAF1A / CAF1 Antibodies

Anti-CHAF1A / CAF1 Antibody (clone SS 1 1-13) IHC-plus™ LS-B242
Mouse Monoclonal [clone SS 1 1-13] (IgG1) to Human CHAF1A / CAF1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-CHAF1A / CAF1 Antibody (aa21-120, AP) LS-C276253
Mouse Monoclonal (IgG2b) to Human CHAF1A / CAF1
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Western blot, ELISA
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Anti-CHAF1A / CAF1 Antibody (aa21-120, FITC) LS-C276256
Mouse Monoclonal (IgG2b) to Human CHAF1A / CAF1
Human
Western blot, ELISA
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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone SS 1 1-13] (IgG1) to Human CHAF1A / CAF1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human CHAF1A / CAF1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [SS 1 1-13]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation

Specificity and Use

CHAF1A / CAF1 antibody was raised against recombinant human CAF-1p150
Specific for CAF-1p150.
Immunohistochemistry: LS-B242 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B242 was determined to be 1:50.

Packaging

Ascites, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CHAF1A / CAF1

Q13111 NM_005483 NP_005474.2

CHAF1A Antibody, CAF-I p150 Antibody, CAF1P150 Antibody, CAF-1 subunit A Antibody, CAF-1 Antibody, Hp150 Antibody, p150 Antibody, CAF Antibody, CAF-I 150 kDa subunit Antibody, CAF1 Antibody, CAF1B Antibody

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4.

Immunohistochemistry

Immunohistochemistry
Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
 

Requested From: United States
Date Requested: 12/5/2016

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