Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
CFLAR / FLIP antibody was raised against peptide at C-terminus of FLIPL.
Recognizes the 55kD FLIPL protein, a FLICE inhibitory protein. FLIP was cloned independently in a number of laboratories and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP has two death effector domains (DEDs) and a caspase-like domain. It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors. FLIP exists in a short form (FLIPS) and a long form (FLIPL). Only FLIPL is recognized by this antibody.
Western Blot: 1:500-1:2000.
PBS, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.