Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CFLAR / FLIP antibody was raised against peptide at C-terminus of FLIPL.
Recognizes the 55kD FLIPL protein, a FLICE inhibitory protein. FLIP was cloned independently in a number of laboratories and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP has two death effector domains (DEDs) and a caspase-like domain. It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors. FLIP exists in a short form (FLIPS) and a long form (FLIPL). Only FLIPL is recognized by this antibody.
Western Blot: 1:500-1:2000.
PBS, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.